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The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells

Human pluripotent stem cells (hPSC) are known to acquire chromosomal abnormalities, which range from point mutations to large copy number changes, including full chromosome aneuploidy. These aberrations have a wide-ranging influence on the state of cells, in both the undifferentiated and differentia...

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Detalles Bibliográficos
Autores principales: Keller, Alexander, Spits, Claudia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625075/
https://www.ncbi.nlm.nih.gov/pubmed/34831467
http://dx.doi.org/10.3390/cells10113246
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author Keller, Alexander
Spits, Claudia
author_facet Keller, Alexander
Spits, Claudia
author_sort Keller, Alexander
collection PubMed
description Human pluripotent stem cells (hPSC) are known to acquire chromosomal abnormalities, which range from point mutations to large copy number changes, including full chromosome aneuploidy. These aberrations have a wide-ranging influence on the state of cells, in both the undifferentiated and differentiated state. Currently, very little is known on how these abnormalities will impact the clinical translation of hPSC, and particularly their potential to prime cells for oncogenic transformation. A further complication is that many of these abnormalities exist in a mosaic state in culture, which complicates their detection with conventional karyotyping methods. In this review we discuss current knowledge on how these aberrations influence the cell state and how this may impact the future of research and the cells’ clinical potential.
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spelling pubmed-86250752021-11-27 The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells Keller, Alexander Spits, Claudia Cells Review Human pluripotent stem cells (hPSC) are known to acquire chromosomal abnormalities, which range from point mutations to large copy number changes, including full chromosome aneuploidy. These aberrations have a wide-ranging influence on the state of cells, in both the undifferentiated and differentiated state. Currently, very little is known on how these abnormalities will impact the clinical translation of hPSC, and particularly their potential to prime cells for oncogenic transformation. A further complication is that many of these abnormalities exist in a mosaic state in culture, which complicates their detection with conventional karyotyping methods. In this review we discuss current knowledge on how these aberrations influence the cell state and how this may impact the future of research and the cells’ clinical potential. MDPI 2021-11-19 /pmc/articles/PMC8625075/ /pubmed/34831467 http://dx.doi.org/10.3390/cells10113246 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Keller, Alexander
Spits, Claudia
The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title_full The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title_fullStr The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title_full_unstemmed The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title_short The Impact of Acquired Genetic Abnormalities on the Clinical Translation of Human Pluripotent Stem Cells
title_sort impact of acquired genetic abnormalities on the clinical translation of human pluripotent stem cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8625075/
https://www.ncbi.nlm.nih.gov/pubmed/34831467
http://dx.doi.org/10.3390/cells10113246
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