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Salt-Mediated Organic Solvent Precipitation for Enhanced Recovery of Peptides Generated by Pepsin Digestion

Conventional solvent-based precipitation makes it challenging to obtain a high recovery of low mass peptides. However, we previously demonstrated that the inclusion of salt ions, specifically ZnSO(4), together with high concentrations of acetone, maximizes the recovery of peptides generated from try...

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Detalles Bibliográficos
Autores principales: Baghalabadi, Venus, Razmi, Habib, Doucette, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8628918/
https://www.ncbi.nlm.nih.gov/pubmed/34842819
http://dx.doi.org/10.3390/proteomes9040044