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Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples
Quantifying pathogenic genes with q-PCR in complex samples to determine the pathogen loads is influenced by a wide range of factors, including choice of extraction method, standard curve, and the decision to use relative versus absolute quantification of the genes. The aim was to investigate the sta...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629182/ https://www.ncbi.nlm.nih.gov/pubmed/34843501 http://dx.doi.org/10.1371/journal.pone.0260082 |