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Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples

Quantifying pathogenic genes with q-PCR in complex samples to determine the pathogen loads is influenced by a wide range of factors, including choice of extraction method, standard curve, and the decision to use relative versus absolute quantification of the genes. The aim was to investigate the sta...

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Detalles Bibliográficos
Autores principales: Hoorzook, K. B., Barnard, T. G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629182/
https://www.ncbi.nlm.nih.gov/pubmed/34843501
http://dx.doi.org/10.1371/journal.pone.0260082