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Absolute quantification of E. coli virulence and housekeeping genes to determine pathogen loads in enumerated environmental samples
Quantifying pathogenic genes with q-PCR in complex samples to determine the pathogen loads is influenced by a wide range of factors, including choice of extraction method, standard curve, and the decision to use relative versus absolute quantification of the genes. The aim was to investigate the sta...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8629182/ https://www.ncbi.nlm.nih.gov/pubmed/34843501 http://dx.doi.org/10.1371/journal.pone.0260082 |