Optimization of a fluorescent-mRNA based real-time assay for precise kinetic measurements of ribosomal translocation

Kinetic characterization of ribosomal translocation is important for understanding the mechanism of elongation in protein synthesis. Here we have optimized a popular fluorescent-mRNA based translocation assay conducted in stopped-flow, by calibrating it with the functional tripeptide formation assay...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Changil, Holm, Mikael, Mandava, Chandra Sekhar, Sanyal, Suparna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632105/
https://www.ncbi.nlm.nih.gov/pubmed/33938388
http://dx.doi.org/10.1080/15476286.2021.1913312

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632105/
https://www.ncbi.nlm.nih.gov/pubmed/33938388
http://dx.doi.org/10.1080/15476286.2021.1913312

Ejemplares similares