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Optimization of a fluorescent-mRNA based real-time assay for precise kinetic measurements of ribosomal translocation
Kinetic characterization of ribosomal translocation is important for understanding the mechanism of elongation in protein synthesis. Here we have optimized a popular fluorescent-mRNA based translocation assay conducted in stopped-flow, by calibrating it with the functional tripeptide formation assay...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Taylor & Francis
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632105/ https://www.ncbi.nlm.nih.gov/pubmed/33938388 http://dx.doi.org/10.1080/15476286.2021.1913312 |