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KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics

Indigenous peoples of the Americas are proficient in botanical medicine. KCNQ family voltage-gated potassium (Kv) channels are sensitive to a variety of ligands, including plant metabolites. Here, we screened methanolic extracts prepared from 40 Californian coastal redwood forest plants for effects...

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Autores principales: Abbott, Geoffrey W., Redford, Kaitlyn E., Yoshimura, Ryan F., Manville, Rían W., Moreira, Luiz, Tran, Kevin, Arena, Grey, Kookootsedes, Alexandra, Lasky, Emma, Gunnison, Elliot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632246/
https://www.ncbi.nlm.nih.gov/pubmed/34858215
http://dx.doi.org/10.3389/fphys.2021.777057
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author Abbott, Geoffrey W.
Redford, Kaitlyn E.
Yoshimura, Ryan F.
Manville, Rían W.
Moreira, Luiz
Tran, Kevin
Arena, Grey
Kookootsedes, Alexandra
Lasky, Emma
Gunnison, Elliot
author_facet Abbott, Geoffrey W.
Redford, Kaitlyn E.
Yoshimura, Ryan F.
Manville, Rían W.
Moreira, Luiz
Tran, Kevin
Arena, Grey
Kookootsedes, Alexandra
Lasky, Emma
Gunnison, Elliot
author_sort Abbott, Geoffrey W.
collection PubMed
description Indigenous peoples of the Americas are proficient in botanical medicine. KCNQ family voltage-gated potassium (Kv) channels are sensitive to a variety of ligands, including plant metabolites. Here, we screened methanolic extracts prepared from 40 Californian coastal redwood forest plants for effects on Kv current and membrane potential in Xenopus oocytes heterologously expressing KCNQ2/3, which regulates excitability of neurons, including those that sense pain. Extracts from 9 of the 40 plant species increased KCNQ2/3 current at –60 mV by ≥threefold (maximally, 15-fold by Urtica dioica) and/or hyperpolarized membrane potential by ≥-3 mV (maximally, –11 mV by Arctostaphylos glandulosa). All nine plants have traditionally been used as both analgesics and gastrointestinal therapeutics. Of two extracts tested, both acted as KCNQ-dependent analgesics in mice. KCNQ2/3 activation at physiologically relevant, subthreshold membrane potentials by tannic acid, gallic acid and quercetin provided molecular correlates for analgesic action of several of the plants. While tannic acid also activated KCNQ1 and KCNQ1-KCNE1 at hyperpolarized, negative membrane potentials, it inhibited KCNQ1-KCNE3 at both negative and positive membrane potentials, mechanistically rationalizing historical use of tannic acid-containing plants as gastrointestinal therapeutics. KCNE dependence of KCNQ channel modulation by plant metabolites therefore provides a molecular mechanistic basis for Native American use of specific plants as both analgesics and gastrointestinal aids.
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spelling pubmed-86322462021-12-01 KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics Abbott, Geoffrey W. Redford, Kaitlyn E. Yoshimura, Ryan F. Manville, Rían W. Moreira, Luiz Tran, Kevin Arena, Grey Kookootsedes, Alexandra Lasky, Emma Gunnison, Elliot Front Physiol Physiology Indigenous peoples of the Americas are proficient in botanical medicine. KCNQ family voltage-gated potassium (Kv) channels are sensitive to a variety of ligands, including plant metabolites. Here, we screened methanolic extracts prepared from 40 Californian coastal redwood forest plants for effects on Kv current and membrane potential in Xenopus oocytes heterologously expressing KCNQ2/3, which regulates excitability of neurons, including those that sense pain. Extracts from 9 of the 40 plant species increased KCNQ2/3 current at –60 mV by ≥threefold (maximally, 15-fold by Urtica dioica) and/or hyperpolarized membrane potential by ≥-3 mV (maximally, –11 mV by Arctostaphylos glandulosa). All nine plants have traditionally been used as both analgesics and gastrointestinal therapeutics. Of two extracts tested, both acted as KCNQ-dependent analgesics in mice. KCNQ2/3 activation at physiologically relevant, subthreshold membrane potentials by tannic acid, gallic acid and quercetin provided molecular correlates for analgesic action of several of the plants. While tannic acid also activated KCNQ1 and KCNQ1-KCNE1 at hyperpolarized, negative membrane potentials, it inhibited KCNQ1-KCNE3 at both negative and positive membrane potentials, mechanistically rationalizing historical use of tannic acid-containing plants as gastrointestinal therapeutics. KCNE dependence of KCNQ channel modulation by plant metabolites therefore provides a molecular mechanistic basis for Native American use of specific plants as both analgesics and gastrointestinal aids. Frontiers Media S.A. 2021-11-11 /pmc/articles/PMC8632246/ /pubmed/34858215 http://dx.doi.org/10.3389/fphys.2021.777057 Text en Copyright © 2021 Abbott, Redford, Yoshimura, Manville, Moreira, Tran, Arena, Kookootsedes, Lasky and Gunnison. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Abbott, Geoffrey W.
Redford, Kaitlyn E.
Yoshimura, Ryan F.
Manville, Rían W.
Moreira, Luiz
Tran, Kevin
Arena, Grey
Kookootsedes, Alexandra
Lasky, Emma
Gunnison, Elliot
KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title_full KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title_fullStr KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title_full_unstemmed KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title_short KCNQ and KCNE Isoform-Dependent Pharmacology Rationalizes Native American Dual Use of Specific Plants as Both Analgesics and Gastrointestinal Therapeutics
title_sort kcnq and kcne isoform-dependent pharmacology rationalizes native american dual use of specific plants as both analgesics and gastrointestinal therapeutics
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632246/
https://www.ncbi.nlm.nih.gov/pubmed/34858215
http://dx.doi.org/10.3389/fphys.2021.777057
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