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Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics
BACKGROUND: Polygonum Multiflorum Radix Preparata (PMP), prepared from Polygonum multiflorum Thunb. (PM), is traditionally valued for its liver and kidney-tonifying effects. However, the previous studies showed that PMP was hepatotoxic, which limited its clinical use. Unfortunately, the potential he...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632464/ https://www.ncbi.nlm.nih.gov/pubmed/34858511 http://dx.doi.org/10.1155/2021/7677392 |
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author | Wang, Liming Wang, Zhida Xing, Yanchao Liu, Erwei Gao, Xiumei Wang, Linlin Fu, Zhifei |
author_facet | Wang, Liming Wang, Zhida Xing, Yanchao Liu, Erwei Gao, Xiumei Wang, Linlin Fu, Zhifei |
author_sort | Wang, Liming |
collection | PubMed |
description | BACKGROUND: Polygonum Multiflorum Radix Preparata (PMP), prepared from Polygonum multiflorum Thunb. (PM), is traditionally valued for its liver and kidney-tonifying effects. However, the previous studies showed that PMP was hepatotoxic, which limited its clinical use. Unfortunately, the potential hepatotoxic ingredients and the molecular mechanism are still uncertain. OBJECTIVE: The aim of this study was to find out potential biomarkers of hepatotoxicity using metabolomics profile. MATERIALS AND METHODS: 60% ethanol extract of PMP (PMPE) was prepared. Subsequently, an untargeted metabolomics technology in combination with ROC curve analysis method was applied to investigate the alteration of plasma metabolites in rats after oral administration of PMPE (40 g/kg/d) for 28 days. RESULTS: Compared to the control group, the significant difference in metabolic profiling was observed in the PMPE-induced liver injury group, and sixteen highly specific biomarkers were identified. These metabolites were mainly enriched into bile acids, lipids, and energy metabolisms, indicating that PMPE-induced liver injury could be related to cholestasis and dysregulated lipid metabolism. CONCLUSIONS: This study is contributed to understand the potential pathogenesis of PMP-induced liver injury. The metabonomic method may be a valuable tool for the clinical diagnosis of PMP-induced liver injury. |
format | Online Article Text |
id | pubmed-8632464 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-86324642021-12-01 Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics Wang, Liming Wang, Zhida Xing, Yanchao Liu, Erwei Gao, Xiumei Wang, Linlin Fu, Zhifei Evid Based Complement Alternat Med Research Article BACKGROUND: Polygonum Multiflorum Radix Preparata (PMP), prepared from Polygonum multiflorum Thunb. (PM), is traditionally valued for its liver and kidney-tonifying effects. However, the previous studies showed that PMP was hepatotoxic, which limited its clinical use. Unfortunately, the potential hepatotoxic ingredients and the molecular mechanism are still uncertain. OBJECTIVE: The aim of this study was to find out potential biomarkers of hepatotoxicity using metabolomics profile. MATERIALS AND METHODS: 60% ethanol extract of PMP (PMPE) was prepared. Subsequently, an untargeted metabolomics technology in combination with ROC curve analysis method was applied to investigate the alteration of plasma metabolites in rats after oral administration of PMPE (40 g/kg/d) for 28 days. RESULTS: Compared to the control group, the significant difference in metabolic profiling was observed in the PMPE-induced liver injury group, and sixteen highly specific biomarkers were identified. These metabolites were mainly enriched into bile acids, lipids, and energy metabolisms, indicating that PMPE-induced liver injury could be related to cholestasis and dysregulated lipid metabolism. CONCLUSIONS: This study is contributed to understand the potential pathogenesis of PMP-induced liver injury. The metabonomic method may be a valuable tool for the clinical diagnosis of PMP-induced liver injury. Hindawi 2021-11-23 /pmc/articles/PMC8632464/ /pubmed/34858511 http://dx.doi.org/10.1155/2021/7677392 Text en Copyright © 2021 Liming Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Liming Wang, Zhida Xing, Yanchao Liu, Erwei Gao, Xiumei Wang, Linlin Fu, Zhifei Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title | Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title_full | Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title_fullStr | Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title_full_unstemmed | Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title_short | Biomarkers and Mechanism Analysis for Polygoni Multiflori Radix Preparata-Induced Liver Injury by UHPLC-Q-TOF-MS-Based Metabolomics |
title_sort | biomarkers and mechanism analysis for polygoni multiflori radix preparata-induced liver injury by uhplc-q-tof-ms-based metabolomics |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8632464/ https://www.ncbi.nlm.nih.gov/pubmed/34858511 http://dx.doi.org/10.1155/2021/7677392 |
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