Dissociation of intact adult mouse cortical projection neurons for single-cell RNA-seq

This protocol provides an improved pipeline for dissociating intact projection neurons from adult mouse cortex for applications including droplet and plate-based single-cell RNA sequencing, qPCR, immunocytochemistry, and long-term in vitro cell culture. This protocol provides a robust and reproducib...

Descripción completa

Detalles Bibliográficos
Autores principales: Golan, Noa, Cafferty, William B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633369/
https://www.ncbi.nlm.nih.gov/pubmed/34877546
http://dx.doi.org/10.1016/j.xpro.2021.100941

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633369/
https://www.ncbi.nlm.nih.gov/pubmed/34877546
http://dx.doi.org/10.1016/j.xpro.2021.100941

Ejemplares similares