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Dissociation of intact adult mouse cortical projection neurons for single-cell RNA-seq
This protocol provides an improved pipeline for dissociating intact projection neurons from adult mouse cortex for applications including droplet and plate-based single-cell RNA sequencing, qPCR, immunocytochemistry, and long-term in vitro cell culture. This protocol provides a robust and reproducib...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633369/ https://www.ncbi.nlm.nih.gov/pubmed/34877546 http://dx.doi.org/10.1016/j.xpro.2021.100941 |