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Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells
Endocytic internalization of extracellular proteins plays roles in signaling, nutrient uptake, immunity, and extracellular protein quality control. However, there are few protocols for analyzing the lysosomal degradation of extracellular protein. Here, we purified secreted proteins fused with pH-sen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633370/ https://www.ncbi.nlm.nih.gov/pubmed/34877548 http://dx.doi.org/10.1016/j.xpro.2021.100975 |