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Protocol for quantification of the lysosomal degradation of extracellular proteins into mammalian cells

Endocytic internalization of extracellular proteins plays roles in signaling, nutrient uptake, immunity, and extracellular protein quality control. However, there are few protocols for analyzing the lysosomal degradation of extracellular protein. Here, we purified secreted proteins fused with pH-sen...

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Detalles Bibliográficos
Autores principales: Tomihari, Ayaka, Chiba, Momoka, Matsuura, Akira, Itakura, Eisuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633370/
https://www.ncbi.nlm.nih.gov/pubmed/34877548
http://dx.doi.org/10.1016/j.xpro.2021.100975