A recombineering pipeline to clone large and complex genes in Chlamydomonas

The ability to clone genes has greatly advanced cell and molecular biology research, enabling researchers to generate fluorescent protein fusions for localization and confirm genetic causation by mutant complementation. Most gene cloning is polymerase chain reaction (PCR)�or DNA synthesis-dependent,...

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Detalles Bibliográficos
Autores principales: Emrich-Mills, Tom Z, Yates, Gary, Barrett, James, Girr, Philipp, Grouneva, Irina, Lau, Chun Sing, Walker, Charlotte E, Kwok, Tsz Kam, Davey, John W, Johnson, Matthew P, Mackinder, Luke C M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Regular Issue
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633747/
https://www.ncbi.nlm.nih.gov/pubmed/33723601
http://dx.doi.org/10.1093/plcell/koab024

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8633747/
https://www.ncbi.nlm.nih.gov/pubmed/33723601
http://dx.doi.org/10.1093/plcell/koab024

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