Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells

DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microscopy-based...

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Detalles Bibliográficos
Autores principales: Kilgas, Susan, Kiltie, Anne E., Ramadan, Kristijan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634038/
https://www.ncbi.nlm.nih.gov/pubmed/34888531
http://dx.doi.org/10.1016/j.xpro.2021.100978

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634038/
https://www.ncbi.nlm.nih.gov/pubmed/34888531
http://dx.doi.org/10.1016/j.xpro.2021.100978

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