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Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro

Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53(+) cells (by 40.2%) induced by exposure to H(2)O(2) (60 μM). The cytopr...

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Autores principales: Sazonova, E. N., Lebed’ko, O. A., Pinaeva, O. G., Tsimbalist, N. A., Kupriyanova, D. A., Tarasov, P. K., Malofey, Yu. B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636574/
https://www.ncbi.nlm.nih.gov/pubmed/34855076
http://dx.doi.org/10.1007/s10517-021-05374-2
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author Sazonova, E. N.
Lebed’ko, O. A.
Pinaeva, O. G.
Tsimbalist, N. A.
Kupriyanova, D. A.
Tarasov, P. K.
Malofey, Yu. B.
author_facet Sazonova, E. N.
Lebed’ko, O. A.
Pinaeva, O. G.
Tsimbalist, N. A.
Kupriyanova, D. A.
Tarasov, P. K.
Malofey, Yu. B.
author_sort Sazonova, E. N.
collection PubMed
description Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53(+) cells (by 40.2%) induced by exposure to H(2)O(2) (60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53(+) cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of H(2)O(2): the number of p53(+) cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE.
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spelling pubmed-86365742021-12-02 Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro Sazonova, E. N. Lebed’ko, O. A. Pinaeva, O. G. Tsimbalist, N. A. Kupriyanova, D. A. Tarasov, P. K. Malofey, Yu. B. Bull Exp Biol Med Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine) Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53(+) cells (by 40.2%) induced by exposure to H(2)O(2) (60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53(+) cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of H(2)O(2): the number of p53(+) cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE. Springer US 2021-12-02 2021 /pmc/articles/PMC8636574/ /pubmed/34855076 http://dx.doi.org/10.1007/s10517-021-05374-2 Text en © Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Sazonova, E. N.
Lebed’ko, O. A.
Pinaeva, O. G.
Tsimbalist, N. A.
Kupriyanova, D. A.
Tarasov, P. K.
Malofey, Yu. B.
Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title_full Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title_fullStr Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title_full_unstemmed Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title_short Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro
title_sort role of amino acid arginine and nitric oxide in mechanisms of cytoprotective effect of non-opiate leu-enkephalin analogue in vitro
topic Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636574/
https://www.ncbi.nlm.nih.gov/pubmed/34855076
http://dx.doi.org/10.1007/s10517-021-05374-2
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