Cargando…

Kinetic and structural mechanism for DNA unwinding by a non-hexameric helicase

UvrD, a model for non-hexameric Superfamily 1 helicases, utilizes ATP hydrolysis to translocate stepwise along single-stranded DNA and unwind the duplex. Previous estimates of its step size have been indirect, and a consensus on its stepping mechanism is lacking. To dissect the mechanism underlying...

Descripción completa

Detalles Bibliográficos
Autores principales:	Carney, Sean P., Ma, Wen, Whitley, Kevin D., Jia, Haifeng, Lohman, Timothy M., Luthey-Schulten, Zaida, Chemla, Yann R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8636605/ https://www.ncbi.nlm.nih.gov/pubmed/34853304 http://dx.doi.org/10.1038/s41467-021-27304-6

Ejemplares similares

Free-energy simulations reveal molecular mechanism for functional switch of a DNA helicase
por: Ma, Wen, et al.
Publicado: (2018)

Regulation of Rep helicase unwinding by an auto-inhibitory subdomain
por: Makurath, Monika A, et al.
Publicado: (2019)

Structural Mechanisms of Hexameric Helicase Loading, Assembly, and Unwinding
por: Trakselis, Michael A.
Publicado: (2016)

Unwinding of a DNA replication fork by a hexameric viral helicase
por: Javed, Abid, et al.
Publicado: (2021)

Hexameric helicase G40P unwinds DNA in single base pair steps
por: Schlierf, Michael, et al.
Publicado: (2019)

A flexible brace maintains the assembly of a hexameric replicative helicase during DNA unwinding
por: Whelan, Fiona, et al.
Publicado: (2012)

Sequence-dependent base pair stepping dynamics in XPD helicase unwinding
por: Qi, Zhi, et al.
Publicado: (2013)

Mechanism of DNA unwinding by hexameric MCM8–9 in complex with HROB
por: Acharya, Ananya, et al.
Publicado: (2023)

ATPase Cycle and DNA Unwinding Kinetics of RecG Helicase
por: Toseland, Christopher P., et al.
Publicado: (2012)

The mechanism of DNA unwinding by the eukaryotic replicative helicase
por: Burnham, Daniel R., et al.
Publicado: (2019)

DNA unwinding mechanism of a eukaryotic replicative CMG helicase
por: Yuan, Zuanning, et al.
Publicado: (2020)

Quantitative and kinetic single-molecule analysis of DNA unwinding by Escherichia coli UvrD helicase
por: Yokota, Hiroaki
Publicado: (2022)

Visualizing helicases unwinding DNA at the single molecule level
por: Fili, Natali, et al.
Publicado: (2010)

DNA unwinding and protein displacement by superfamily 1 and superfamily 2 helicases
por: Mackintosh, Samuel G., et al.
Publicado: (2006)

Mutations Altering the Interplay between GkDnaC Helicase and DNA Reveal an Insight into Helicase Unwinding
por: Lo, Yu-Hua, et al.
Publicado: (2011)

Structural basis for DNA strand separation by a hexameric replicative helicase
por: Chaban, Yuriy, et al.
Publicado: (2015)

Dynamic structural insights into the molecular mechanism of DNA unwinding by the bacteriophage T7 helicase
por: Ma, Jian-Bing, et al.
Publicado: (2020)

Unwinding forward and sliding back: an intermittent unwinding mode of the BLM helicase
por: Wang, Shuang, et al.
Publicado: (2015)

Correction: Mutations Altering the Interplay between GkDnaC Helicase and DNA Reveal an Insight into Helicase Unwinding
por: Lo, Yu-Hua, et al.
Publicado: (2012)

Unwinding and Rewinding: Double Faces of Helicase?
por: Wu, Yuliang
Publicado: (2012)

Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases
por: Su, Nannan, et al.
Publicado: (2019)

The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA
por: Gai, Dahai, et al.
Publicado: (2016)

The hexameric structure of the human mitochondrial replicative helicase Twinkle
por: Fernández-Millán, Pablo, et al.
Publicado: (2015)

Active DNA unwinding and transport by a membrane-adapted helicase nanopore
por: Sun, Ke, et al.
Publicado: (2019)

The convergence of head-on DNA unwinding forks induces helicase oligomerization and activity transition
por: Bi, Lulu, et al.
Publicado: (2022)

Unwinding the roles of RNA helicase MOV10
por: Nawaz, Aatiqa, et al.
Publicado: (2021)

Retraction: The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA
por: Gai, Dahai, et al.
Publicado: (2019)

Functional Dynamics of Hexameric Helicase Probed by Hydrogen Exchange and Simulation
por: Radou, Gaël, et al.
Publicado: (2014)

Mechanism of Nucleic Acid Unwinding by SARS-CoV Helicase
por: Adedeji, Adeyemi O., et al.
Publicado: (2012)

The yeast Pif1p DNA helicase preferentially unwinds RNA–DNA substrates
por: Boulé, Jean-Baptiste, et al.
Publicado: (2007)

Duplex DNA engagement and RPA oppositely regulate the DNA-unwinding rate of CMG helicase
por: Kose, Hazal B., et al.
Publicado: (2020)

The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA
por: Lo, Yu-Hua, et al.
Publicado: (2009)

Evidence for a functional dimeric form of the PcrA helicase in DNA unwinding
por: Yang, Ye, et al.
Publicado: (2008)

Molecular characteristics of reiterative DNA unwinding by the Caenorhabditis elegans RecQ helicase
por: Choi, Seoyun, et al.
Publicado: (2019)

Ring-shaped replicative helicase encircles double-stranded DNA during unwinding
por: Joo, Sihwa, et al.
Publicado: (2019)

Human Pif1 helicase unwinds synthetic DNA structures resembling stalled DNA replication forks
por: George, Tresa, et al.
Publicado: (2009)

Phosphate release contributes to the rate-limiting step for unwinding by an RNA helicase
por: Wang, Qixin, et al.
Publicado: (2010)

ssDNA reeling is an intermediate step in the reiterative DNA unwinding activity of the WRN-1 helicase
por: Le, Son Truong, et al.
Publicado: (2023)

Single-Molecule Fluorescence Reveals the Unwinding Stepping Mechanism of Replicative Helicase
por: Syed, Salman, et al.
Publicado: (2014)

Insights into the mechanism of a G-quadruplex-unwinding DEAH-box helicase
por: Chen, Michael C., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_44am71gpnprohdj88obfabc8r2