A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopy

We report a rapid experimental procedure based on high-density in vivo psoralen inter-strand DNA cross-linking coupled to spreading of naked purified DNA, positive staining, low-angle rotary shadowing, and transmission electron microscopy (TEM) that allows quick visualization of the dynamic of heavy...

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Detalles Bibliográficos
Autores principales: Kosar, Martin, Piccini, Daniele, Foiani, Marco, Giannattasio, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643652/
https://www.ncbi.nlm.nih.gov/pubmed/34500456
http://dx.doi.org/10.1093/nar/gkab770

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643652/
https://www.ncbi.nlm.nih.gov/pubmed/34500456
http://dx.doi.org/10.1093/nar/gkab770

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