Cargando…

A rapid method to visualize human mitochondrial DNA replication through rotary shadowing and transmission electron microscopy

We report a rapid experimental procedure based on high-density in vivo psoralen inter-strand DNA cross-linking coupled to spreading of naked purified DNA, positive staining, low-angle rotary shadowing, and transmission electron microscopy (TEM) that allows quick visualization of the dynamic of heavy...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kosar, Martin, Piccini, Daniele, Foiani, Marco, Giannattasio, Michele
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643652/ https://www.ncbi.nlm.nih.gov/pubmed/34500456 http://dx.doi.org/10.1093/nar/gkab770

Ejemplares similares

Measurement of replication structures at the nanometer scale using super-resolution light microscopy
por: Baddeley, D., et al.
Publicado: (2010)

Rapid high-resolution measurement of DNA replication timing by droplet digital PCR
por: Batrakou, Dzmitry G, et al.
Publicado: (2018)

MitoZ: a toolkit for animal mitochondrial genome assembly, annotation and visualization
por: Meng, Guanliang, et al.
Publicado: (2019)

Visualization of uracils created by APOBEC3A using UdgX shows colocalization with RPA at stalled replication forks
por: Stewart, Jessica A, et al.
Publicado: (2020)

3D reconstruction and comparison of shapes of DNA minicircles observed by cryo-electron microscopy
por: Amzallag, Arnaud, et al.
Publicado: (2006)

Rapid interactome profiling by massive sequencing
por: Di Niro, Roberto, et al.
Publicado: (2010)

Selection of DNA aptamers using atomic force microscopy
por: Miyachi, Yusuke, et al.
Publicado: (2010)

The dynamics of genome replication using deep sequencing
por: Müller, Carolin A., et al.
Publicado: (2014)

Nonparametric expression analysis using inferential replicate counts
por: Zhu, Anqi, et al.
Publicado: (2019)

Direct observation of damage clustering in irradiated DNA with atomic force microscopy
por: Xu, Xu, et al.
Publicado: (2020)

DNA molecular combing-based replication fork directionality profiling
por: Blin, Marion, et al.
Publicado: (2021)

Multiplexed single-cell profiling of chromatin states at genomic loci by expansion microscopy
por: Woodworth, Marcus A, et al.
Publicado: (2021)

Expansion microscopy allows high resolution single cell analysis of epigenetic readers
por: Acke, Aline, et al.
Publicado: (2022)

Adenovirus vector-mediated assay system for hepatitis C virus replication
por: Yoshida, Takeshi, et al.
Publicado: (2011)

Real-time single-molecule observation of rolling-circle DNA replication
por: Tanner, Nathan A., et al.
Publicado: (2009)

Differential peak calling of ChIP-seq signals with replicates with THOR
por: Allhoff, Manuel, et al.
Publicado: (2016)

Detection of base analogs incorporated during DNA replication by nanopore sequencing
por: Georgieva, Daniela, et al.
Publicado: (2020)

Analysis of scanning force microscopy images of protein-induced DNA bending using simulations
por: Dame, Remus T., et al.
Publicado: (2005)

Imaging chromatin nanostructure with binding-activated localization microscopy based on DNA structure fluctuations
por: Szczurek, Aleksander, et al.
Publicado: (2017)

Scoring schemes of palindrome clusters for more sensitive prediction of replication origins in herpesviruses
por: Chew, David S. H., et al.
Publicado: (2005)

Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis
por: Takahashi, Nobuhiro, et al.
Publicado: (2005)

Monitoring DNA replication in fission yeast by incorporation of 5-ethynyl-2′-deoxyuridine
por: Hua, Hui, et al.
Publicado: (2011)

Synthetic riboswitches for external regulation of genes transferred by replication-deficient and oncolytic adenoviruses
por: Ketzer, Patrick, et al.
Publicado: (2012)

OKseqHMM: a genome-wide replication fork directionality analysis toolkit
por: Liu, Yaqun, et al.
Publicado: (2023)

Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy
por: Etheridge, Thomas J., et al.
Publicado: (2014)

Measurement of incorporation kinetics of non-fluorescent native nucleotides by DNA polymerases using fluorescence microscopy
por: Walsh, Matthew T., et al.
Publicado: (2017)

Two-dimensional intact mitochondrial DNA agarose electrophoresis reveals the structural complexity of the mammalian mitochondrial genome
por: Kolesar, Jill E., et al.
Publicado: (2013)

p53-dependent antiviral RNA-interference facilitates tumor-selective viral replication
por: Gürlevik, Engin, et al.
Publicado: (2009)

Nucleic acid visualization with UCSF Chimera
por: Couch, Gregory S., et al.
Publicado: (2006)

Single cell epigenetic visualization assay
por: Kint, Sam, et al.
Publicado: (2021)

Rapid one-step recombinational cloning
por: Fu, Changlin, et al.
Publicado: (2008)

RATT: Rapid Annotation Transfer Tool
por: Otto, Thomas D., et al.
Publicado: (2011)

De novo assembly of genuine replication forks on an immobilized circular plasmid in Xenopus egg extracts
por: Zembutsu, Akiko, et al.
Publicado: (2006)

Genome-wide copy number profiling of single cells in S-phase reveals DNA-replication domains
por: Van der Aa, Niels, et al.
Publicado: (2013)

Detection of differentially methylated regions from whole-genome bisulfite sequencing data without replicates
por: Wu, Hao, et al.
Publicado: (2015)

A proteinase-free DNA replication machinery for in vitro and in vivo amplified MicroRNA imaging
por: Wei, Jie, et al.
Publicado: (2020)

aniFOUND: analysing the associated proteome and genomic landscape of the repaired nascent non-replicative chromatin
por: Stefos, Georgios C, et al.
Publicado: (2021)

Visualizing single-stranded nucleic acids in solution
por: Plumridge, Alex, et al.
Publicado: (2017)

Molecule-level imaging of Pax6 mRNA distribution in mouse embryonic neocortex by molecular interaction force microscopy
por: Jung, Yu Jin, et al.
Publicado: (2009)

Label-free, atomic force microscopy-based mapping of DNA intrinsic curvature for the nanoscale comparative analysis of bent duplexes
por: Buzio, Renato, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_nur50pd4aor156g8kq0d21nv4a