Probing the stability of the SpCas9–DNA complex after cleavage

CRISPR–Cas9 is a ribonucleoprotein complex that sequence-specifically binds and cleaves double-stranded DNA. Wildtype Cas9 and its nickase and cleavage-incompetent mutants have been used in various biological techniques due to their versatility and programmable specificity. Cas9 has been shown to bi...

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Detalles Bibliográficos
Autores principales: Aldag, Pierre, Welzel, Fabian, Jakob, Leonhard, Schmidbauer, Andreas, Rutkauskas, Marius, Fettes, Fergus, Grohmann, Dina, Seidel, Ralf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643700/
https://www.ncbi.nlm.nih.gov/pubmed/34792162
http://dx.doi.org/10.1093/nar/gkab1072

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8643700/
https://www.ncbi.nlm.nih.gov/pubmed/34792162
http://dx.doi.org/10.1093/nar/gkab1072

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