CoolTip: Low-Temperature Solid-Phase Extraction Microcolumn for Capturing Hydrophilic Peptides and Phosphopeptides

Reversed-phase solid-phase extraction (SPE) techniques are commonly used for desalting samples before LC/MS/MS in shotgun proteomics. However, hydrophilic peptides are often lost during the desalting step under the standard SPE conditions. Here, we describe a simple protocol in which a stop-and-go e...

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Detalles Bibliográficos
Autores principales: Ogata, Kosuke, Ishihama, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Technological Innovation and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646264/
https://www.ncbi.nlm.nih.gov/pubmed/34740827
http://dx.doi.org/10.1016/j.mcpro.2021.100170

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8646264/
https://www.ncbi.nlm.nih.gov/pubmed/34740827
http://dx.doi.org/10.1016/j.mcpro.2021.100170

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