Preparation of mouse pancreatic tumor for single-cell RNA sequencing and analysis of the data

Preparation of single-cell suspension from primary tumor tissue can provide a valuable resource for functional, genetic, proteomic, and tumor microenvironment studies. Here, we describe an effective protocol for mouse pancreatic tumor dissociation with further processing of tumor suspension for sing...

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Detalles Bibliográficos
Autores principales: Surumbayeva, Aizhan, Kotliar, Michael, Gabitova-Cornell, Linara, Kartashov, Andrey, Peri, Suraj, Salomonis, Nathan, Barski, Artem, Astsaturov, Igor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649395/
https://www.ncbi.nlm.nih.gov/pubmed/34927097
http://dx.doi.org/10.1016/j.xpro.2021.100989

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649395/
https://www.ncbi.nlm.nih.gov/pubmed/34927097
http://dx.doi.org/10.1016/j.xpro.2021.100989

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