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Global Run-On sequencing to measure nascent transcription in C. elegans

Global Run-On sequencing (GRO-seq) is one of the most sensitive techniques to detect nascent transcription from RNA polymerase (Pol) at a genome-wide level. The protocol incorporates labeled ribonucleotides into nascent RNAs from Pol I, II, and III. We have adapted the GRO-seq protocol to the nemato...

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Detalles Bibliográficos
Autores principales: Quarato, Piergiuseppe, Cecere, Germano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8649397/
https://www.ncbi.nlm.nih.gov/pubmed/34927098
http://dx.doi.org/10.1016/j.xpro.2021.100991