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Synthesis and application of POLYseq for profiling human liver organoids

Detailed herein is the protocol for synthesis, characterization, and application of POLYseq for cell pooling in single-cell sequencing runs. POLYseq is synthesized through commercially available reagents and is highly tailorable. Synthesis is easily performed in a two-step protocol, utilizing Michae...

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Detalles Bibliográficos
Autores principales: Dunn, Andrew W., Cai, Yuqi, Iwasawa, Kentaro, Kimura, Masaki, Takebe, Takanori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654628/
https://www.ncbi.nlm.nih.gov/pubmed/34934958
http://dx.doi.org/10.1016/j.xpro.2021.100976