Cargando…
Synthesis and application of POLYseq for profiling human liver organoids
Detailed herein is the protocol for synthesis, characterization, and application of POLYseq for cell pooling in single-cell sequencing runs. POLYseq is synthesized through commercially available reagents and is highly tailorable. Synthesis is easily performed in a two-step protocol, utilizing Michae...
Autores principales: | Dunn, Andrew W., Cai, Yuqi, Iwasawa, Kentaro, Kimura, Masaki, Takebe, Takanori |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654628/ https://www.ncbi.nlm.nih.gov/pubmed/34934958 http://dx.doi.org/10.1016/j.xpro.2021.100976 |
Ejemplares similares
-
POLYseq: A poly(β-amino ester)-based vector for multifunctional cellular barcoding
por: Dunn, Andrew, et al.
Publicado: (2021) -
Synthetic augmentation of bilirubin metabolism in human pluripotent stem cell-derived liver organoids
por: Reza, Hasan Al, et al.
Publicado: (2023) -
Digitalized Human Organoid for Wireless Phenotyping
por: Kimura, Masaki, et al.
Publicado: (2018) -
A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis
por: Kang, Soo-Yeon, et al.
Publicado: (2023) -
Protocol to profile the bioenergetics of organoids using Seahorse
por: Ludikhuize, Marlies C., et al.
Publicado: (2021)