Targeted insertion of large genetic payloads using cas directed LINE-1 reverse transcriptase

A difficult genome editing goal is the site-specific insertion of large genetic constructs. Here we describe the GENEWRITE system, where site-specific targetable activity of Cas endonucleases is coupled with the reverse transcriptase activity of the ORF2p protein of the human retrotransposon LINE-1....

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Detalles Bibliográficos
Autores principales: Manoj, Femila, Tai, Laura W., Wang, Katelyn Sun Mi, Kuhlman, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654924/
https://www.ncbi.nlm.nih.gov/pubmed/34880381
http://dx.doi.org/10.1038/s41598-021-03130-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654924/
https://www.ncbi.nlm.nih.gov/pubmed/34880381
http://dx.doi.org/10.1038/s41598-021-03130-0

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