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Targeted insertion of large genetic payloads using cas directed LINE-1 reverse transcriptase
A difficult genome editing goal is the site-specific insertion of large genetic constructs. Here we describe the GENEWRITE system, where site-specific targetable activity of Cas endonucleases is coupled with the reverse transcriptase activity of the ORF2p protein of the human retrotransposon LINE-1....
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654924/ https://www.ncbi.nlm.nih.gov/pubmed/34880381 http://dx.doi.org/10.1038/s41598-021-03130-0 |