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Detecting in-solution conformational changes in viral fusogens using tryptophan-induced fluorescence quenching

Dynamic monitoring of protein conformational changes is necessary to fully understand many biological processes. For example, viral entry and membrane fusion require rearrangement of its viral glycoprotein. We present a step-by-step protocol for site-specific bimane labeling of the influenza-C fusog...

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Detalles Bibliográficos
Autores principales: Serrão, Vitor Hugo B., Lee, Jeffrey E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654978/
https://www.ncbi.nlm.nih.gov/pubmed/34934961
http://dx.doi.org/10.1016/j.xpro.2021.100994