Cargando…
Detecting in-solution conformational changes in viral fusogens using tryptophan-induced fluorescence quenching
Dynamic monitoring of protein conformational changes is necessary to fully understand many biological processes. For example, viral entry and membrane fusion require rearrangement of its viral glycoprotein. We present a step-by-step protocol for site-specific bimane labeling of the influenza-C fusog...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654978/ https://www.ncbi.nlm.nih.gov/pubmed/34934961 http://dx.doi.org/10.1016/j.xpro.2021.100994 |