Ultrastructural localization of de novo synthesized phosphatidylcholine in yeast cells by freeze-fracture electron microscopy

Phosphatidylcholine (PtdCho) is a major membrane phospholipid synthesized in the endoplasmic reticulum. Here, we provide a protocol using electron microscopy to localize PtdCho that is newly synthesized by the Kennedy pathway in yeast cells. The protocol consists of the administration of a clickable...

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Detalles Bibliográficos
Autores principales: Tsuji, Takuma, Fujimoto, Toyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654986/
https://www.ncbi.nlm.nih.gov/pubmed/34934959
http://dx.doi.org/10.1016/j.xpro.2021.100990

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8654986/
https://www.ncbi.nlm.nih.gov/pubmed/34934959
http://dx.doi.org/10.1016/j.xpro.2021.100990

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