High-throughput nanofluidic real-time PCR to discriminate Pneumococcal Conjugate Vaccine (PCV)-associated serogroups 6, 18, and 22 to serotypes using modified oligonucleotides

Current real-time high-throughput Polymerase Chain Reaction (qPCR) methods do not distinguish serotypes 6A from 6B, 18C from 18A/B and 22F from 22A. We established a nanofluidic real-time PCR (Fluidigm) for serotyping that included Dual-Priming-Oligonucleotides (DPO), a Locked-Nucleic-Acid (LNA) pro...

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Detalles Bibliográficos
Autores principales: Downs, S. L., Madhi, S. A., Van der Merwe, L., Nunes, M. C., Olwagen, C. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660885/
https://www.ncbi.nlm.nih.gov/pubmed/34887480
http://dx.doi.org/10.1038/s41598-021-03127-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8660885/
https://www.ncbi.nlm.nih.gov/pubmed/34887480
http://dx.doi.org/10.1038/s41598-021-03127-9

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