ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is primarily transmitted through droplets. All human tissues with the angiotensin‐converting enzyme 2 (ACE2) and transmembrane protease serines 2 (TRMPRSS2) are potential targets of SARS‐CoV‐2. The role of saliva in SARS‐CoV‐2 transmission...

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Autores principales: Zhu, Fucun, Zhong, Yi, Ji, Huan, Ge, Ran, Guo, Lu, Song, Haiyang, Wu, Heming, Jiao, Pengfei, Li, Sheng, Wang, Chenxing, Du, Hongming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662096/
https://www.ncbi.nlm.nih.gov/pubmed/34590312
http://dx.doi.org/10.1111/joa.13560
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author Zhu, Fucun
Zhong, Yi
Ji, Huan
Ge, Ran
Guo, Lu
Song, Haiyang
Wu, Heming
Jiao, Pengfei
Li, Sheng
Wang, Chenxing
Du, Hongming
author_facet Zhu, Fucun
Zhong, Yi
Ji, Huan
Ge, Ran
Guo, Lu
Song, Haiyang
Wu, Heming
Jiao, Pengfei
Li, Sheng
Wang, Chenxing
Du, Hongming
author_sort Zhu, Fucun
collection PubMed
description Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is primarily transmitted through droplets. All human tissues with the angiotensin‐converting enzyme 2 (ACE2) and transmembrane protease serines 2 (TRMPRSS2) are potential targets of SARS‐CoV‐2. The role of saliva in SARS‐CoV‐2 transmission remains obscure. In this study, we attempted to reveal ACE2 and TRMPRSS2 protein expression in human parotid, submandibular, and sublingual glands (three major salivary glands). Then, the binding function of spike protein to ACE2 in three major salivary glands was detected. The expression of ACE2 and TMPRSS2 in human saliva from parotid glands were both examined. Exogenous recombined ACE2 and TMPRSS2 anchoring and fusing to oral mucosal epithelial cells in vitro were also unraveled. ACE2 and TMPRSS2 were found mainly to be expressed in the cytomembrane and cytoplasm of epithelial cells in the serous acinus cells in parotid and submandibular glands. Our research also discovered that the spike protein of SARS‐CoV‐2 binds to ACE2 in salivary glands in vitro. Furthermore, exogenous ACE2 and TMPRSS2 can anchor and fuse to oral mucosa in vitro. Thus, the expression of ACE2 and TMPRSS2 in human saliva might have implications for SARS‐CoV‐2 infection.
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spelling pubmed-86620962021-12-10 ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium Zhu, Fucun Zhong, Yi Ji, Huan Ge, Ran Guo, Lu Song, Haiyang Wu, Heming Jiao, Pengfei Li, Sheng Wang, Chenxing Du, Hongming J Anat Original Papers Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is primarily transmitted through droplets. All human tissues with the angiotensin‐converting enzyme 2 (ACE2) and transmembrane protease serines 2 (TRMPRSS2) are potential targets of SARS‐CoV‐2. The role of saliva in SARS‐CoV‐2 transmission remains obscure. In this study, we attempted to reveal ACE2 and TRMPRSS2 protein expression in human parotid, submandibular, and sublingual glands (three major salivary glands). Then, the binding function of spike protein to ACE2 in three major salivary glands was detected. The expression of ACE2 and TMPRSS2 in human saliva from parotid glands were both examined. Exogenous recombined ACE2 and TMPRSS2 anchoring and fusing to oral mucosal epithelial cells in vitro were also unraveled. ACE2 and TMPRSS2 were found mainly to be expressed in the cytomembrane and cytoplasm of epithelial cells in the serous acinus cells in parotid and submandibular glands. Our research also discovered that the spike protein of SARS‐CoV‐2 binds to ACE2 in salivary glands in vitro. Furthermore, exogenous ACE2 and TMPRSS2 can anchor and fuse to oral mucosa in vitro. Thus, the expression of ACE2 and TMPRSS2 in human saliva might have implications for SARS‐CoV‐2 infection. John Wiley and Sons Inc. 2021-09-29 2022-02 /pmc/articles/PMC8662096/ /pubmed/34590312 http://dx.doi.org/10.1111/joa.13560 Text en © 2021 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Papers
Zhu, Fucun
Zhong, Yi
Ji, Huan
Ge, Ran
Guo, Lu
Song, Haiyang
Wu, Heming
Jiao, Pengfei
Li, Sheng
Wang, Chenxing
Du, Hongming
ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title_full ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title_fullStr ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title_full_unstemmed ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title_short ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium
title_sort ace2 and tmprss2 in human saliva can adsorb to the oral mucosal epithelium
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8662096/
https://www.ncbi.nlm.nih.gov/pubmed/34590312
http://dx.doi.org/10.1111/joa.13560
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