Cargando…

A far-red light–inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation

The CRISPR-Cas12a has been harnessed as a powerful tool for manipulating targeted gene expression. The possibility to manipulate the activity of CRISPR-Cas12a with a more precise spatiotemporal resolution and deep tissue permeability will enable targeted genome engineering and deepen our understandi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Xinyi, Dong, Kaili, Kong, Deqiang, Zhou, Yang, Yin, Jianli, Cai, Fengfeng, Wang, Meiyan, Ye, Haifeng
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Association for the Advancement of Science 2021
Materias:	Biomedicine and Life Sciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8664267/ https://www.ncbi.nlm.nih.gov/pubmed/34890237 http://dx.doi.org/10.1126/sciadv.abh2358

Ejemplares similares

A highly specific CRISPR-Cas12j nuclease enables allele-specific genome editing
por: Wang, Yao, et al.
Publicado: (2023)

Hierarchical self-uncloaking CRISPR-Cas13a–customized RNA nanococoons for spatial-controlled genome editing and precise cancer therapy
por: Fan, Ningke, et al.
Publicado: (2022)

Genome-editing prodrug: Targeted delivery and conditional stabilization of CRISPR-Cas9 for precision therapy of inflammatory disease
por: Yan, Xiaojie, et al.
Publicado: (2021)

Comprehensive analysis and accurate quantification of unintended large gene modifications induced by CRISPR-Cas9 gene editing
por: Park, So Hyun, et al.
Publicado: (2022)

Efficient cancer modeling through CRISPR-Cas9/HDR-based somatic precision gene editing in mice
por: Bu, Wen, et al.
Publicado: (2023)

In vivo delivery of CRISPR-Cas9 using lipid nanoparticles enables antithrombin gene editing for sustainable hemophilia A and B therapy
por: Han, Jeong Pil, et al.
Publicado: (2022)

Helical allophycocyanin nanotubes absorb far-red light in a thermophilic cyanobacterium
por: Gisriel, Christopher J., et al.
Publicado: (2023)

Targeted gene silencing in the nervous system with CRISPR-Cas13
por: Powell, Jackson E., et al.
Publicado: (2022)

Analysis of bacterial pangenomes reduces CRISPR dark matter and reveals strong association between membranome and CRISPR-Cas systems
por: Rubio, Alejandro, et al.
Publicado: (2023)

Changes in supramolecular organization of cyanobacterial thylakoid membrane complexes in response to far-red light photoacclimation
por: MacGregor-Chatwin, Craig, et al.
Publicado: (2022)

An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
por: Hansen, Stine L., et al.
Publicado: (2023)

Blood-brain barrier–penetrating single CRISPR-Cas9 nanocapsules for effective and safe glioblastoma gene therapy
por: Zou, Yan, et al.
Publicado: (2022)

A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila
por: Doll, Roman M., et al.
Publicado: (2023)

Host nucleases generate prespacers for primed adaptation in the E. coli type I-E CRISPR-Cas system
por: Shiriaeva, Anna A., et al.
Publicado: (2022)

Precise CRISPR-Cas–mediated gene repair with minimal off-target and unintended on-target mutations in human hematopoietic stem cells
por: Tran, Ngoc Tung, et al.
Publicado: (2022)

Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation
por: Shao, Jiawei, et al.
Publicado: (2018)

CRISPR-Cas9 screen reveals a role of purine synthesis for estrogen receptor α activity and tamoxifen resistance of breast cancer cells
por: Hany, Dina, et al.
Publicado: (2023)

A universal bacteriophage T4 nanoparticle platform to design multiplex SARS-CoV-2 vaccine candidates by CRISPR engineering
por: Zhu, Jingen, et al.
Publicado: (2021)

CRISPR-based engineering of RNA viruses
por: Nemudryi, Artem, et al.
Publicado: (2023)

Liver cancer heterogeneity modeled by in situ genome editing of hepatocytes
por: Tang, Mei, et al.
Publicado: (2022)

Cloning and base editing of GFP transgenic rhesus monkey and off-target analysis
por: Kang, Yu, et al.
Publicado: (2022)

Application of combined CRISPR screening for genetic and chemical-genetic interaction profiling in Mycobacterium tuberculosis
por: Yan, Mei-Yi, et al.
Publicado: (2022)

Optogenetic modeling of human neuromuscular circuits in Duchenne muscular dystrophy with CRISPR and pharmacological corrections
por: Paredes-Redondo, Amaia, et al.
Publicado: (2021)

Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors
por: Yu, Yuanhuan, et al.
Publicado: (2020)

Genome-wide CRISPR screens using isogenic cells reveal vulnerabilities conferred by loss of tumor suppressors
por: Feng, Xu, et al.
Publicado: (2022)

A programmable high-expression yeast platform responsive to user-defined signals
por: Liu, Qi, et al.
Publicado: (2022)

CancerVar: An artificial intelligence–empowered platform for clinical interpretation of somatic mutations in cancer
por: Li, Quan, et al.
Publicado: (2022)

A human mucosal melanoma organoid platform for modeling tumor heterogeneity and exploring immunotherapy combination options
por: Sun, Lulu, et al.
Publicado: (2023)

Novel suction-based in vivo cutaneous DNA transfection platform
por: Lallow, Emran O., et al.
Publicado: (2021)

Clinically translatable cytokine delivery platform for eradication of intraperitoneal tumors
por: Nash, Amanda M., et al.
Publicado: (2022)

Discovery of integrons in Archaea: Platforms for cross-domain gene transfer
por: Ghaly, Timothy M., et al.
Publicado: (2022)

In vivo multidimensional CRISPR screens identify Lgals2 as an immunotherapy target in triple-negative breast cancer
por: Ji, Peng, et al.
Publicado: (2022)

Exosome-mediated delivery of Cas9 ribonucleoprotein complexes for tissue-specific gene therapy of liver diseases
por: Wan, Tao, et al.
Publicado: (2022)

Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells
por: Gu, Yuanzhuo, et al.
Publicado: (2023)

The transmembrane endoplasmic reticulum–associated E3 ubiquitin ligase TRIM13 restrains the pathogenic-DNA–triggered inflammatory response
por: Li, Xuelian, et al.
Publicado: (2022)

Autoinhibition and activation mechanisms revealed by the triangular-shaped structure of myosin Va
por: Niu, Fengfeng, et al.
Publicado: (2022)

CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells
por: Werder, Rhiannon B., et al.
Publicado: (2022)

Near-infrared upconversion–activated CRISPR-Cas9 system: A remote-controlled gene editing platform
por: Pan, Yongchun, et al.
Publicado: (2019)

A comprehensive long-read isoform analysis platform and sequencing resource for breast cancer
por: Veiga, Diogo F. T., et al.
Publicado: (2022)

Development of iPSC-based clinical trial selection platform for patients with ultrarare diseases
por: Sequiera, Glen Lester, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_b4r883qlaov88svao0vmtrmh1t