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Nanopore sequencing of single-cell transcriptomes with scCOLOR-seq

Here we describe single-cell corrected long-read sequencing (scCOLOR-seq), which enables error correction of barcode and unique molecular identifier oligonucleotide sequences and permits standalone cDNA nanopore sequencing of single cells. Barcodes and unique molecular identifiers are synthesized us...

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Detalles Bibliográficos
Autores principales: Philpott, Martin, Watson, Jonathan, Thakurta, Anjan, Brown, Tom, Oppermann, Udo, Cribbs, Adam P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8668430/
https://www.ncbi.nlm.nih.gov/pubmed/34211161
http://dx.doi.org/10.1038/s41587-021-00965-w