Thiol profiling in cancer cell lines by HPLC-mass spectrometry

We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify...

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Detalles Bibliográficos
Autores principales: Liu, Xiaoguang, Koppula, Pranavi, Olszewski, Kellen, Gan, Boyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669100/
https://www.ncbi.nlm.nih.gov/pubmed/34917974
http://dx.doi.org/10.1016/j.xpro.2021.100977

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669100/
https://www.ncbi.nlm.nih.gov/pubmed/34917974
http://dx.doi.org/10.1016/j.xpro.2021.100977

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