Thiol profiling in cancer cell lines by HPLC-mass spectrometry

We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify...

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Detalles Bibliográficos
Autores principales: Liu, Xiaoguang, Koppula, Pranavi, Olszewski, Kellen, Gan, Boyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669100/
https://www.ncbi.nlm.nih.gov/pubmed/34917974
http://dx.doi.org/10.1016/j.xpro.2021.100977
Descripción
Sumario:We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).

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