Thiol profiling in cancer cell lines by HPLC-mass spectrometry

We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify...

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Detalles Bibliográficos
Autores principales: Liu, Xiaoguang, Koppula, Pranavi, Olszewski, Kellen, Gan, Boyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669100/
https://www.ncbi.nlm.nih.gov/pubmed/34917974
http://dx.doi.org/10.1016/j.xpro.2021.100977
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author Liu, Xiaoguang
Koppula, Pranavi
Olszewski, Kellen
Gan, Boyi
author_facet Liu, Xiaoguang
Koppula, Pranavi
Olszewski, Kellen
Gan, Boyi
author_sort Liu, Xiaoguang
collection PubMed
description We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021).
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spelling pubmed-86691002021-12-15 Thiol profiling in cancer cell lines by HPLC-mass spectrometry Liu, Xiaoguang Koppula, Pranavi Olszewski, Kellen Gan, Boyi STAR Protoc Protocol We describe a protocol for identifying cellular thiol metabolites such as cysteine and cystine in adherent cells using high performance liquid chromatography (HPLC) tandem mass spectrometry-based metabolomics. We applied a modified extraction and sample derivatization protocol to accurately quantify the intracellular levels of labile thiol species and to inhibit oxidation prior to analysis. For complete details on the use and execution of this protocol, please refer to Liu et al. (2020) and Koppula et al. (2021). Elsevier 2021-12-08 /pmc/articles/PMC8669100/ /pubmed/34917974 http://dx.doi.org/10.1016/j.xpro.2021.100977 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Liu, Xiaoguang
Koppula, Pranavi
Olszewski, Kellen
Gan, Boyi
Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title_full Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title_fullStr Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title_full_unstemmed Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title_short Thiol profiling in cancer cell lines by HPLC-mass spectrometry
title_sort thiol profiling in cancer cell lines by hplc-mass spectrometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669100/
https://www.ncbi.nlm.nih.gov/pubmed/34917974
http://dx.doi.org/10.1016/j.xpro.2021.100977
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AT olszewskikellen thiolprofilingincancercelllinesbyhplcmassspectrometry
AT ganboyi thiolprofilingincancercelllinesbyhplcmassspectrometry

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