ACTB and GAPDH appear at multiple SDS-PAGE positions, thus not suitable as reference genes for determining protein loading in techniques like Western blotting

We performed polyacrylamide gel electrophoresis of human proteins with sodium dodecyl sulfate, isolated proteins at multiple positions, and then used liquid chromatography and tandem mass spectrometry (LC-MS/MS) to determine the protein identities. Although beta-actin (ACTB) and glyceraldehyde-3-pho...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Keyin, Zhang, Ju, Ding, Nan, Zellmer, Lucas, Zhao, Yan, Liu, Siqi, Liao, Dezhong Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669867/
https://www.ncbi.nlm.nih.gov/pubmed/34966852
http://dx.doi.org/10.1515/biol-2021-0130

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8669867/
https://www.ncbi.nlm.nih.gov/pubmed/34966852
http://dx.doi.org/10.1515/biol-2021-0130

Ejemplares similares