Mg(2+)-dependent conformational rearrangements of CRISPR-Cas12a R-loop complex are mandatory for complete double-stranded DNA cleavage

CRISPR-Cas12a, an RNA-guided DNA targeting endonuclease, has been widely used for genome editing and nucleic acid detection. As part of the essential processes for both of these applications, the two strands of double-stranded DNA are sequentially cleaved by a single catalytic site of Cas12a, but th...

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Detalles Bibliográficos
Autores principales: Son, Heyjin, Park, Jaeil, Hwang, Injoo, Jung, Youngri, Bae, Sangsu, Lee, Sanghwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670479/
https://www.ncbi.nlm.nih.gov/pubmed/34853172
http://dx.doi.org/10.1073/pnas.2113747118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8670479/
https://www.ncbi.nlm.nih.gov/pubmed/34853172
http://dx.doi.org/10.1073/pnas.2113747118

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