Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog

We have developed a protocol for barcoded cDNA libraries of 48 samples to study gene expression across tissues in the domestic dog, Canis familiaris, by modifying the Single-Cell Tagged Reverse Transcription (STRT) protocol (Islam et al., 2012, 2014). The cDNA reads represent mRNA 5′ ends, enabling...

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Detalles Bibliográficos
Autores principales: Ezer, Sini, Yoshihara, Masahito, Katayama, Shintaro, Daub, Carsten, Lohi, Hannes, Krjutskov, Kaarel, Kere, Juha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672047/
https://www.ncbi.nlm.nih.gov/pubmed/34950881
http://dx.doi.org/10.1016/j.xpro.2021.100995

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672047/
https://www.ncbi.nlm.nih.gov/pubmed/34950881
http://dx.doi.org/10.1016/j.xpro.2021.100995

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