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Isolation and functional characterization of primary endothelial cells from mouse cerebral cortex

Endothelial cells (ECs) lining blood vessels are implicated in organ development, function, and maintenance. We present a detailed protocol enabling isolation and characterization of primary mouse brain ECs, including quality controls and functional assays. These procedures promote survival of prima...

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Detalles Bibliográficos
Autores principales: Ouellette, Julie, Lacoste, Baptiste
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672097/
https://www.ncbi.nlm.nih.gov/pubmed/34950893
http://dx.doi.org/10.1016/j.xpro.2021.101019