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Isolation and functional characterization of primary endothelial cells from mouse cerebral cortex
Endothelial cells (ECs) lining blood vessels are implicated in organ development, function, and maintenance. We present a detailed protocol enabling isolation and characterization of primary mouse brain ECs, including quality controls and functional assays. These procedures promote survival of prima...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672097/ https://www.ncbi.nlm.nih.gov/pubmed/34950893 http://dx.doi.org/10.1016/j.xpro.2021.101019 |