Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain

Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. H...

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Autores principales: Venturino, Alessandro, Siegert, Sandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672101/
https://www.ncbi.nlm.nih.gov/pubmed/34950889
http://dx.doi.org/10.1016/j.xpro.2021.101012
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author Venturino, Alessandro
Siegert, Sandra
author_facet Venturino, Alessandro
Siegert, Sandra
author_sort Venturino, Alessandro
collection PubMed
description Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021).
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spelling pubmed-86721012021-12-22 Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain Venturino, Alessandro Siegert, Sandra STAR Protoc Protocol Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021). Elsevier 2021-12-11 /pmc/articles/PMC8672101/ /pubmed/34950889 http://dx.doi.org/10.1016/j.xpro.2021.101012 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Venturino, Alessandro
Siegert, Sandra
Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title_full Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title_fullStr Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title_full_unstemmed Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title_short Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
title_sort minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8672101/
https://www.ncbi.nlm.nih.gov/pubmed/34950889
http://dx.doi.org/10.1016/j.xpro.2021.101012
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