A simple and rapid method for enzymatic synthesis of CRISPR-Cas9 sgRNA libraries

CRISPR-Cas9 sgRNA libraries have transformed functional genetic screening and have enabled several innovative methods that rely on simultaneously targeting numerous genetic loci. Such libraries could be used in a vast number of biological systems and in the development of new technologies, but libra...

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Detalles Bibliográficos
Autores principales: Yates, Joshua D, Russell, Robert C, Barton, Nathaniel J, Yost, H Joseph, Hill, Jonathon T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682767/
https://www.ncbi.nlm.nih.gov/pubmed/34554233
http://dx.doi.org/10.1093/nar/gkab838

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682767/
https://www.ncbi.nlm.nih.gov/pubmed/34554233
http://dx.doi.org/10.1093/nar/gkab838

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