Efficient fractionation and analysis of ribosome assembly intermediates in human cells

Biochemical studies of the human ribosome synthesis pathway have been hindered by technical difficulties in obtaining intact preribosomal complexes from internal regions of the nucleolus. Here we provide a detailed description of an extraction method that enables efficient detection, isolation, and...

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Detalles Bibliográficos
Autores principales: Nieto, Blanca, Gaspar, Sonia G., Sapio, Russell T., Clavaín, Laura, Bustelo, Xosé R., Pestov, Dimitri G., Dosil, Mercedes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Technical Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682975/
https://www.ncbi.nlm.nih.gov/pubmed/34530680
http://dx.doi.org/10.1080/15476286.2021.1965754

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682975/
https://www.ncbi.nlm.nih.gov/pubmed/34530680
http://dx.doi.org/10.1080/15476286.2021.1965754

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