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Efficient fractionation and analysis of ribosome assembly intermediates in human cells
Biochemical studies of the human ribosome synthesis pathway have been hindered by technical difficulties in obtaining intact preribosomal complexes from internal regions of the nucleolus. Here we provide a detailed description of an extraction method that enables efficient detection, isolation, and...
Autores principales: | Nieto, Blanca, Gaspar, Sonia G., Sapio, Russell T., Clavaín, Laura, Bustelo, Xosé R., Pestov, Dimitri G., Dosil, Mercedes |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8682975/ https://www.ncbi.nlm.nih.gov/pubmed/34530680 http://dx.doi.org/10.1080/15476286.2021.1965754 |
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