ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples

BACKGROUND: One limiting factor of short amplicon 16S rRNA gene sequencing approaches is the use of low DNA amounts in the amplicon generation step. Especially for low-biomass samples, insufficient or even commonly undetectable DNA amounts can limit or prohibit further analysis in standard protocols...

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Detalles Bibliográficos
Autores principales: Abellan-Schneyder, Isabel, Schusser, Andrea Janina, Neuhaus, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684222/
https://www.ncbi.nlm.nih.gov/pubmed/34922460
http://dx.doi.org/10.1186/s12866-021-02391-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8684222/
https://www.ncbi.nlm.nih.gov/pubmed/34922460
http://dx.doi.org/10.1186/s12866-021-02391-z

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