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Protocol for in vitro analysis of pro-inflammatory and metabolic functions of cultured primary murine astrocytes
Robust protocols are required to investigate in vitro the molecular mechanisms that control astrocyte metabolism and pro-inflammatory activities. In the present protocol, we describe step by step the isolation and culture of primary murine astrocytes from neonatal brains, followed by their genetic m...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8686069/ https://www.ncbi.nlm.nih.gov/pubmed/34977679 http://dx.doi.org/10.1016/j.xpro.2021.101033 |