Cargando…

An optimized genome-wide, virus-free CRISPR screen for mammalian cells

Pooled CRISPR screens have been widely applied to mammalian and other organisms to elucidate the interplay between genes and phenotypes of interest. The most popular method for delivering the CRISPR components into mammalian cells is lentivirus based. However, because lentivirus is not always an opt...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiong, Kai, la Cour Karottki, Karen Julie, Hefzi, Hooman, Li, Songyuan, Grav, Lise Marie, Li, Shangzhong, Spahn, Philipp, Lee, Jae Seong, Ventina, Ildze, Lee, Gyun Min, Lewis, Nathan E., Kildegaard, Helene Faustrup, Pedersen, Lasse Ebdrup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8687118/
https://www.ncbi.nlm.nih.gov/pubmed/34935002
http://dx.doi.org/10.1016/j.crmeth.2021.100062