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Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit

Single biomolecule sensing often requires the quantification of multiple fluorescent species. Here, we theoretically and experimentally use time-resolved fluorescence via Time Correlated Single Photon Counting (TCSPC) to accurately quantify fluorescent species with similar chromatic signatures. A mo...

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Detalles Bibliográficos
Autores principales: Nasser, Maisa, Meller, Amit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689154/
https://www.ncbi.nlm.nih.gov/pubmed/34977508
http://dx.doi.org/10.1016/j.isci.2021.103554