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Lifetime-based analysis of binary fluorophores mixtures in the low photon count limit
Single biomolecule sensing often requires the quantification of multiple fluorescent species. Here, we theoretically and experimentally use time-resolved fluorescence via Time Correlated Single Photon Counting (TCSPC) to accurately quantify fluorescent species with similar chromatic signatures. A mo...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8689154/ https://www.ncbi.nlm.nih.gov/pubmed/34977508 http://dx.doi.org/10.1016/j.isci.2021.103554 |